Lumbar puncture constitutes the simplest method of collecting the cerebrospinal fluid for examination. It is an easy, quick and safe procedure to be applied to most of patients. Lumbar puncture is still commonly use in diagnostics of the nervous system diseases. It provides important data on intracranial pressure and on the composition of the cerebrospinal fluid. It also allows for an introduction of contrast for radiological examination and for intrathecal introduction of drugs.